Immunomagnetic Isolation of Cryptococcus neoformans by Beads Coated with Anti-Cryptococcus Serum
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چکیده
منابع مشابه
Immunomagnetic isolation of Cryptococcus neoformans by beads coated with anti-Cryptococcus serum.
Immunomagnetic separation (IMS) was utilized for the selective isolation of Cryptococcus neoformans from environmental sources, such as soils and pigeon droppings. Magnetic beads coated with anti-cryptococcal IgG (serotypes A and B) were used to isolate the fungus. In a modeled spiking experiment using C. neoformans serotype A strain and anti-serotype A antibody, the recovery rate of the cells ...
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ABSTRACT Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years. Cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (CNS) in immunocompetent as well as immunocompromised patients. We report the development of a semi-nested- PCR-based assay for the ...
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The isolation and identification of Cryptococcus neoformans is improved by use of a potato dextrose medium containing urea-antibiotic supplements and a pH of 3.5.
متن کاملCryptococcus neoformans.
Seven nonencapsulated mutants of Cryptococcus neoformans were isolated from an encapsulated strain of human origin. Initially, the mutants were avirulent for mice. After several months of subculturing, six of the seven isolates reverted LO the encapsulated state and possessed varying degrees of virulence. The results of these experiments suggest that a strong correlation exists between the pres...
متن کاملCryptococcus neoformans: a sugar-coated killer with designer genes.
Cryptococcus neoformans has become a common central nervous system pathogen as the immunocompromised populations enlarge world-wide. This encapsulated yeast has significant advantages for the study of fungal pathogenesis and these include: (1) a clinically important human pathogen; (2) a tractable genetic system; (3) advanced molecular biology foundation; (4) understanding of several virulence ...
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ژورنال
عنوان ژورنال: Nippon Ishinkin Gakkai Zasshi
سال: 2003
ISSN: 0916-4804,1882-0476
DOI: 10.3314/jjmm.44.139